Single Chromosome Sequencing

4DGENOME

4DGenome is a leading Genetic Testing Service,specially in haplotyping technology. We care about the satisfaction of our customers and guarantee top of the line services. Visit us today and see how we can help you or your loved ones.

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SERVICES

haplotyping by single-chromosome sequencing

4DGenome is leading technology company, providing microbiome, single cell, single chromosome, low input whole genome sequencing service. We also offer customized bioinformatics service. Let us provide you with the personalized service you deserve.

WHOLE GENOME SEQUENCING

Low input of DNA or RNA

Whole Genome Sequencing (WGS) provides the most comprehensive map of an organism's genetic information. High quality sequencing is now available with as little as 1 ng of starting material.

SINGLE CHROMOSOME SEQUENCING

Human single chromosome isolation and sequencing

We provide the cutting-edge technology service for single chromosome sequencing. Our novel approach is chromosome range haplotyping determination.

SINGLE CELL SEQUENCING

single cell isolation and whole genome sequencing for DNA or RNA

Single cell sequencing (SCS) is becoming a new approach to study biological heterogeneity. We provide single cell isolation, whole genome amplification and sequencing service.

MICROBIOME SERVICES

16S ribosomal RNA sequencing

We offer 16S ribosomal RNA (rRNA) gene sequencing service and data analysis. Next-generation sequencing enables analysis of the entire microbial community within a sample.

CUSTOMIZED TARGET DESIGN

probe design for DNA capture

Targeted capture provides an efficient and sensitive means for sequencing specific genomic regions in a high-throughput manner. If you’re looking for customized design of your capture sequencing, we will meet your satisfaction.

BIOINFORMATICS SERVICE

High-throughput data analysis

We provide customized service for bioinformatics analysis, including NGS data analysis, haplotyping, imputation and ancestry analysis. We also provide service of software development.

TECHNOLOGY

We isolate single chromosomes and sequence them individually.

ISOLATE ONE CHROMOSOME BY COMPUTER-DIRECTED LASER MICRODISSECTION

ISOLATE THREE SINGLE CHROMOSOMES BY COMPUTER-DIRECTED LASER MICRODISSECTION

AN ILLUSTRATION OF THE GENERAL SEQUENCE PIPELINE

INFORMATICS

SCIENCE&EDUCATION

1. WHAT IS A HAPLOTYPE?

The term haplotype was invented about 50 years ago by the Italian geneticist Ruggero Ceppellini (Milan, 1917 – 1988). Now the term and concept have been widely used since, long before DNA analysis. Human genomes are diploid. Human diploid cells contain two sets of homologous chromosomes, which have slightly different sequences from each other. Their complete description and interpretation require to know not only their mutations but also the arrangement of these mutations onto chromosomal haplotypes. A haplotype is a sequence (or a collection of mutation) of each of those two homologous chromosomes. A haplogroup is a group of similar haplotypes that share common ancestors.

2. WHY NEED CHROMOSOMAL HAPLOTYPES?

It is because two homologous chromosomes have different mutations, are functioning differently, their functionalities are closely related to their combinatorial content of mutations, and their functionalities are closely related to human diseases.

3. WHY NEED TO MEASURE THE HAPLOTYPES EXPERIMENTALLY?

It is because it is much more accurate with less switch errors on long-range or chromosomal-range of haplotyping.

4. WHO NEED HAPLOTYPES?

Disease-gene discovery
Rare diseases and rare mutations
long range eQTLs
Genetic epidemiological studies (association studies, linkage studies)
Functional interpretation of various high-throughput data sets
Linkage disequilibrium assessment
Genetic demography
HLA typing
Ancestry analysis
Evolution
Population history
Pharmaceutical companies
Hospitals
Agriculture

Literature

COMPANY

Get to Know Us a Bit Better

Since its founding in 2014, 4DGenome has been at the forefront of experimental haplotype determination and bioinformatics. Our goal is to offer high-quality products and service to meet your needs on haplotype sequencing and associated bioinformatic analysis. 4DGenome is located in Atlanta, Georgia, about 10 miles north from downtown Atlanta. Quality, satisfaction, and user friendly is our key to operations at 4DGenome.